| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1621 | Alkalihalobacillus clausii | ACAGCACCATGACCGCAA | ACGGCAAGCTTTACGCCA | 59.89 | 60.28 | 285 | 30 |
100.00%
|
100.00%
|
| Primer 1622 | Alkalihalobacillus clausii | ACGATTGGCTGTGGGCTA | AGCACACTCACGAATGCCA | 58.60 | 59.93 | 200 | 30 |
100.00%
|
100.00%
|
| Primer 1623 | Alkalihalobacillus clausii | CGGCAACAGGCTGCAAAT | TCGCCAGTGCCTTGTGTT | 59.35 | 59.81 | 240 | 30 |
100.00%
|
100.00%
|
| Primer 1624 | Alkalihalobacillus clausii | ACGCTTGCAATCGTCGCT | TCGAGGCATTGGCGTGTT | 60.74 | 59.97 | 169 | 30 |
100.00%
|
100.00%
|
| Primer 1625 | Alkalihalobacillus clausii | ATTGTCGTCGCTGAAGGGG | TCATGCCAGCCTTGCCTT | 60.08 | 59.56 | 196 | 30 |
100.00%
|
100.00%
|
| Primer 1626 | Alkalihalobacillus clausii | ATCGGCATGTTGAGCGGT | ATGCCATGGATGACGCCT | 59.73 | 59.08 | 236 | 30 |
100.00%
|
100.00%
|
| Primer 1627 | Alkalihalobacillus clausii | TCGGCATGTTGAGCGGTT | GATGCCATGGATGACGCCT | 59.97 | 60.23 | 236 | 30 |
100.00%
|
100.00%
|
| Primer 1628 | Alkalihalobacillus clausii | AGGGGACACACTGACGGTA | AGCGCTTCTTCGACGGTT | 59.85 | 59.66 | 183 | 30 |
100.00%
|
100.00%
|
| Primer 1629 | Alkalihalobacillus clausii | TGCTCGTGCAGGCATGTT | ACGCTGCGCAAAGGATTG | 60.28 | 59.44 | 190 | 30 |
100.00%
|
100.00%
|
| Primer 1630 | Alkalihalobacillus clausii | TGCTCGTGCAGGCATGTT | GCTGCGCAAAGGATTGGT | 60.28 | 59.04 | 188 | 30 |
100.00%
|
100.00%
|